Amino Acid String Kernels
Exploiting Physico-Chemical Properties in String Kernels
Background: String kernels are commonly used for the classification of biological sequences, nucleotide as well as amino acid sequences. Although string kernels are already very powerful, when it comes to amino acids they have a major short coming. They ignore an important piece of information when comparing amino acids: the physico-chemical properties such as size, hydrophobicity, or charge. This information is very valuable, especially when training data is less abundant. There have been only very few approaches so far that aim at combining these two ideas.
Results: We propose new string kernels that combine the benefits of physico-chemical descriptors for amino acids with the ones of string kernels. The benefits of the proposed kernels are assessed on two problems: MHC-peptide binding classification using position specific kernels and protein classification based on the substring spectrum of the sequences. Our experiments demonstrate that the incorporation of amino acid properties in string kernels yields improved performances compared to standard string kernels and to previously proposed non-substring kernels.
Conclusions: In summary, the proposed modifications, in particular the combination with the RBF substring kernel, consistently yield improvements without affecting the computational complexity. The proposed kernels therefore appear to be the kernels of choice for any protein sequence-based inference.
- Human MHC allele data sets from IEDB benchmark
(retrieved from http://mhcbindingpredictions.immuneepitope.org/dataset.html)
- Protein classification data
(retrieved from http://www.kyb.tuebingen.mpg.de/bs/people/weston/semiprot/supp.html)
- Protein sequences
Plain text table specifying the positive and negative training and test sets for each family. Each row is one sequence, and each column is one family. (0 = not present; 1 = positive train; 2 = negative train; 3 = positive test; 4 = negative test)
- PSIBlast profiles
(retrieved from http://cbio.mskcc.org/leslielab/software/string-kernels)